Vistagen Therapeutics, Inc.

The enclosed proxy is solicited on behalf of the Board of Directors (the Board) of Vistagen Therapeutics, Inc., a Nevada corporation (Vistagen, the Company, us, we orour), for use at Vistagen’s 2026 Annual Meeting of Stockholders (the Annual Meeting or the Meeting). The Annual Meeting will take place in a virtual meeting format on September 10, 2026 at 9:00 am Pacific Daylight Time, and will be held exclusively via the Internet at: https://meetnow.global/M4Y4UQU

To conserve environmental resources and prevent unnecessary corporate expense, we are using the “Notice and Access” method of providing proxy materials to you via the Internet pursuant to the regulations promulgated by the U.S. Securities and Exchange Commission (the SEC). On or about July 31, 2026, we will begin mailing a one-page Notice of Internet Availability of Proxy Materials (the Notice) to each of our stockholders entitled to notice of and to vote at the Annual Meeting. The Notice contains instructions for accessing this Proxy Statement, our Annual Report on SEC Form 10-K for the Company's fiscal year ended March 31, 2026, filed with the SEC on June 15, 2026 (Annual Report), and Annual Meeting voting instructions. The Notice also includes instructions on how you can receive a paper copy of your proxy materials by postal mail. This Proxy Statement and the Annual Report are available on the Internet at: www.envisionreports.com/VTGN