Q32 Bio Inc.

Section 16(a) of the Exchange Act requires our directors and executive officers, and persons who beneficially own more than 10% of a registered class of our equity securities, to file with the SEC initial reports of ownership and reports of changes in ownership of our common stock and other equity securities. Officers, directors and greater than 10% beneficial owners are required by SEC regulations to furnish us with copies of all Section 16(a) forms they file.

To our knowledge, based solely on our review of Forms 3, 4 and 5, and any amendments thereto, filed by such reporting persons and/ or written representations that no Form 5 was required, we believe that during the fiscal year ended December 31, 2024, all filing requirements applicable to our executive officers, directors and persons who beneficially own more than 10% percent of a registered class of our equity securities under the Exchange Act were met in a timely manner except for (i) one transaction by Shelia M. Violette on October 11, 2024 that was inadvertently filed late on October 16, 2024, (ii) one transaction by Isaac Manke on April 1, 2024 that was inadvertently filed late on September 30, 2024, (iii) one transaction by David S. Grayzel on April 1, 2024 that was inadvertently filed late on August 14, 2024, (iv) one transaction by Arthur Tzianabos on March 25, 2024 that was inadvertently filed late on August 14, 2024, (v) one transaction by Paul Alloway on March 25, 2024 that was inadvertently filed late on April 23, 2024, and (vi) one transaction by Charles Michaud Jr. on March 25, 2024 that was inadvertently filed late on April 23, 2024. The transactions were not reported timely due to administrative oversight.