Bolt Biotherapeutics, Inc.

UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

SCHEDULE 14A

Proxy Statement Pursuant to Section 14(a) of the

Securities Exchange Act of 1934

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Filed by a party other than the Registrant ☐

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Preliminary Proxy Statement

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Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e)(2))

☒ Definitive Proxy Statement

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Definitive Additional Materials

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Soliciting Material under §240.14a-12

Bolt Biotherapeutics, Inc.

(Name of Registrant as Specified In Its Charter)

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Fee paid previously with preliminary materials.

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Fee computed on table in exhibit required by Item 25(b) per Exchange Act Rules 14a-6(i)(1) and 0-11.

900 Chesapeake Drive

Redwood City, California 94063

NOTICE OF ANNUAL MEETING OF STOCKHOLDERS

TO BE HELD ON JUNE 10, 2026

AT 11:00 A.M. PACIFIC TIME

Dear Stockholder:

You are cordially invited to attend the 2026 Annual Meeting of Stockholders of Bolt Biotherapeutics, Inc., a Delaware corporation, or the Company or Bolt Bio. The 2026 Annual Meeting of Stockholders will be held on June 10, 2026, at 11:00 a.m., Pacific Time. The 2026 Annual Meeting of Stockholders will be convened and held entirely online. You will be able to attend and participate online in the 2026 Annual Meeting of Stockholders by visitingwww.proxydocs.com/BOLT, where you will be able to listen to the meeting live, submit questions, and vote.

The 2026 Annual Meeting of Stockholders is being convened to conduct the following business:

To elect our two nominees for Class II directors to serve until our 2029 Annual Meeting of Stockholders;

To ratify the selection by the audit committee of the Board of Directors of PricewaterhouseCoopers LLP as our independent registered public accounting firm for the year ending December 31, 2026; and

To conduct any other business properly brought before the 2026 Annual Meeting of Stockholders.

These items of business are more fully described in the Proxy Statement accompanying this Notice of Annual Meeting of Stockholders. The record date for the 2026 Annual Meeting of Stockholders is April 22, 2026. Only stockholders of record at the close of business on that date are entitled to receive notice of, and to vote at, the 2026 Annual Meeting of Stockholders or any adjournment thereof. A list of stockholders of record at the close of business on the April 22, 2026 record date will be available for the 10 days ending the day prior to the Annual Meeting to registered stockholders for any legally valid purpose related to the Annual Meeting. For access to the stockholder list, please contact us at investors@boltbio.com.

The Board of Directors recommends that you vote as follows on the matters to be presented to stockholders at the 2026 Annual Meeting of Stockholders:

FORthe election of each of our Class II director nominees named in Proposal No. 1 of the proxy statement; and

FORthe ratification of the selection by the audit committee of the Board of Directors of PricewaterhouseCoopers LLP, as our independent registered public accounting firm, as described in Proposal No. 2 of the proxy statement.

Your vote is very important. Whether or not you attend the 2026 Annual Meeting of Stockholders (by logging intowww.proxydocs.com/BOLT), it is important that your shares be represented. We encourage you to read the accompanying Proxy Statement and our Annual Report on Form 10-K for the year ended December 31, 2025, and submit your proxy on the internet, by phone or by mail in accordance with the instructions in the Notice of Internet Availability of Proxy Materials or your proxy materials. Please review the instructions on the enclosed proxy card or the information forwarded by your bank, broker or other holder of record regarding each of these voting options. If you receive more than one Notice of Internet Availability of Proxy Materials or full set of proxy materials, your shares are either registered in more than one name or held in different accounts. Please vote the shares covered by each Notice of Internet Availability of Proxy Materials or proxy card. Instructions on how to attend the meeting webcast, ask questions or vote your shares online will also be included with the Notice of Internet Availability of Proxy Materials, and are provided in the Proxy Statement accompanying this Notice of Annual Meeting of Stockholders.

Important Notice Regarding the Availability of Proxy Materials for the Annual Meeting of Stockholders to Be Held on Wednesday, June 10, 2026 electronically via webcast at www.proxydocs.com/BOLT.

This Proxy Statement and our Annual Report on Form 10-K for the year ended December 31, 2025

are available free of charge atwww.proxydocs.com/BOLT.

On behalf of the Board of Directors, thank you for your participation in this important annual process.

By Order of the Board of Directors

/s/ William P. Quinn

William P. QuinnPresident, Chief Executive Officer, Chief Financial Officer and SecretaryRedwood City, California

April 28, 2026

You are cordially invited to attend the 2026 Annual Meeting of Stockholders by logging intowww.proxydocs.com/BOLT and following the attendance instructions on the website. Whether or not you expect to attend the 2026 Annual Meeting of Stockholders, please vote on the internet, by phone or by mail as instructed in the Notice of Internet Availability of Proxy Materials or your proxy materials, as promptly as possible in order to ensure your representation at the meeting. Even if you have voted by proxy prior to the meeting, you may still attend the meeting online and submit your vote prior to voting being closed atwww.proxydocs.com/BOLT. Please note, however, that if you are a beneficial stockholder and hold our stock in the name of a broker, bank or other nominee and you wish to vote at the 2026 Annual Meeting of Stockholders, you must obtain a proxy issued in your name from that record holder.

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